Andreadou Eleni
Andreadou Eleni
Title: Bacterial DING proteins in Alzheimer’s disease and Mild Cognitive Impairment and correlation with Αβ-42 and COX-1/2
Biography
Biography: Andreadou Eleni
Abstract
Alzheimer’s disease (AD) and Mild Cognitive Impairment (MCI) have been linked to inflammation as their pathology is partially attributed to common bacterial infections, while the enormous economic burden of dementia imposes the discovery of new biomarkers in biological fluids other than cerebrospinal fluid (CSF). DING proteins identified mainly by their N-terminal sequences are common in Pseudomonas, have potential roles in phosphate acquisition and pathogenicity and they have been implicated with many human diseases. The aim of this research was to investigate the levels of the bacterial DING proteins in blood serum and their correlation with the neurodegeneration markers Αβ42 and tau and the inflammation markers COX-1 and COX-2. Levels of DING were measured with indirect ELISA in blood serum of AD (N=18) and MCI patients (N=23) in comparison with cognitive healthy individuals (N=13). The biomarkers of inflammation COX-1 and COX-2, and the established in CSF AD biomarkers, Αβ42 and tau, were measured as well. DING levels were found significantly increased in serum of AD patients in comparison with cognitive healthy subjects and MCI patients. Serum DING proteins positively correlate with Αβ42, COX-1 and especially with COX-2 levels. Also, serum DING levels negatively correlate with the mental state of patients. Multi-linear regression analysis proves that DING levels in serum are significantly determined by the mental state of patients and COX-2 levels, as well as the age of patients. AUC analysis proves that serum DING could be used as a possible biomarker in serum to discriminate AD patients from both cognitive healthy individuals and especially MCI patients